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Image Search Results
Journal: Oncotarget
Article Title: Desirable cytolytic immune effector cell recruitment by interleukin-15 dendritic cells
doi: 10.18632/oncotarget.14622
Figure Lengend Snippet: IL-15 DCs superiorly attract cytotoxic immune effector cells ( A ). Floating bars (min to max, line at mean) represent % granzyme B+ cells in PBMC (white bar), PBMC exposed for 3 hours to 48-hour wash-out supernatant of IL-15 DCs (striped dark grey bar) or IL-4 DCs (striped light grey bar), and PBMC following IL-15 DC- (dark grey bar) or IL-4 DC-mediated (light grey bar) migration ( n = 3). ( B ) Representation of % granzyme B+ cells (+ SD) within the different effector cell subsets in full PBMC fraction (white bars) and following IL15 DC- (dark grey bars) or IL-4 DC-mediated (light grey bars) migration ( n = 8). ( C ) Cytotoxicity was determined of migrated purified γδ T cells and NK cells towards 48-hour wash-out supernatant of IL-15 DCs (IL-15 DCs) or IL4 DCs (IL-4 DCs). Non-migrated purified cells (-) were used to define control killing capacity. Daudi and K562 target cells were added at an E:T ratio of 5:1 γδ T cells and 1:1 NK cells, respectively. Percentage tumor cell killing was determined by Annexin-V/PI staining after 4 hours and calculated using the formula specified in “Materials and methods”. Donors are represented by unique symbols ( n = 6). Repeated measures ANOVA with Bonferroni›s Multiple Comparison Test. *** p < 0.001, ** p < 0.01, * p < 0.05, ns, p > 0.5.
Article Snippet: The
Techniques: Migration, Purification, Control, Staining, Comparison